This method can help to demonstrate the differences between circulating T-follicular helper cell subsets in healthy and diseased states.The main advantages of this technique are that it's fast, that the samples can be batch-processed, and that the
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Here, a protocol for the isolation and characterization of CD4+ T-cell subsets from human peripheral blood is described. Purified CD4+ T-cells are analyzed by flow cytometry to determine proportions of T-follicular helper cell subsets.