Results: Determination of the Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node, and Spleen
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Conclusion
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This protocol helps in investigating the role of immune cells in particular organs in health and pathology. The main advantage of this technique is that this is a cost-effective technique since it's a manual technique. Demonstrating the procedure
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Herein, we present a protocol to prepare single cells from murine thymus, pancreatic draining lymph node and spleen to further study these cells using flow cytometry. In addition, this protocol was used for determining the subsets of regulatory T cells using flow cytometry.