Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
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Conclusion
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Our protocol helps investigators to implement standardized experimental pipelines for performing phenotypic characterization of similar populations by immunofluorescent staining. With this technique, researchers can rigorously analyze the similar
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We propose a standardized protocol to characterize the cellular composition of late-stage murine atherosclerotic lesions including systematic methods of animal dissection, tissue embedding, sectioning, staining, and analysis of brachiocephalic arteries from atheroprone smooth muscle cell lineage tracing mice.