Results: Representative Leaf Damage and Gene Expression
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Conclusion
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This protocol uses a streamlined approach for measuring plant gene expression in response to insect herbivory. By using detached leaves isolated in Petri dishes, the insects are easier to apply and monitor during the relatively short period of inf
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The presented method creates natural herbivore damaged plant tissue through the application of Manduca sexta larvae to detached leaves of potato. The plant tissue is assayed for expression of six transcription factor homologs involved in early responses to insect herbivory.