Multiplex fluorescent immunohistochemistry identifies multiple cell types and their locations in intact formalin-fixed paraffin-embedded tissue, allowing not only for cell identification but also cell to cell spatial analysis. The main advantage o
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Multiplex fluorescent immunohistochemistry is an emerging technology that enables the visualization of multiple cell types within intact formalin-fixed, paraffin embedded (FFPE) tissue. Presented are guidelines for ensuring a successful 7-color multiplex with instructions for optimizing antibodies and reagents, preparing slides, design and tips for avoiding common problems.