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Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

9.1K Views

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09:15 min

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July 26th, 2019

DOI :

10.3791/59915-v

July 26th, 2019

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9,063 Views

Jenny Lazarus¹, Yagiz Akiska¹, Mirna Perusina Lanfranca¹, Lawrence Delrosario¹, Lei Sun¹, Daniel Long², Jiaqi Shi³, Howard Crawford², Marina P. Di Magliano¹, Weiping Zou¹, Timothy Frankel¹

¹Department of Surgery, University of Michigan, ²Department of Molecular and Cellular Physiology, University of Michigan, ³Department of Pathology, University of Michigan

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Résumé

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Multiplex Fluorescent Immunohistochemistry

Cell Identification

Tumor Microenvironment

Deparaffinizing

Antigen Retrieval

Primary Antibody

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