Results: Effects of PDGF Treatment on ECM Formation and Endothelial Cell Activation
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Conclusion
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This protocol make it possible to see different types of cultures and observe their interactions with extra-cellular matrix. The main advantage of this method is that the matrix you obtain is regenerated by fibroblast and it will be quite similar
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The aim of this method is to obtain fibroblast-derived 3D matrices as a natural scaffold for subsequent cellular assays. Fibroblasts are seeded in a pre-treated culture plate and stimulated with ascorbic acid for matrix generation. Matrices are decellularized and blocked to culture relevant cells (e.g., endothelial cells).