Placing Multi-Channel Probe in Ex Vivo Brain Tissue Slice
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Patch Clamping Targeted Neurons and Obtaining Whole-Cell Configuration
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Results: Intracellular and Multi-Channel Extracellular Recordings in Cortical Slice
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Conclusion
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This protocol can be used to investigate whether volatile anesthetics disrupts some synopsis more than others, to determine if their effects are cell type or as pathway specific, and to determine what effects occur at the population level. The mai
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Ex vivo brain slices can be used to study the effects of volatile anesthetics on evoked responses to afferent inputs. Optogenetics are employed to independently activate thalamocortical and corticocortical afferents to non-primary neocortex, and synaptic and network responses are modulated with isoflurane.