Results: Representative Calcified Aortic Valve T Cell Isolation and Analysis
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Conclusion
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This protocol is easy to perform. The cloning phase allows a larger number of T clones to be obtained from fresh samples. Moreover, the phenotype and function of T cells can easily be analyzed.
Demonstrating the procedure will be Mary Christ
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In this study, we describe the process of T lymphocyte isolation from fresh samples of calcified aortic valves and the analytical steps of T cell-cloning for the characterization of the adaptive leukocyte subsets by using flow cytometry analysis.