Frster Resonance Energy Transfer (FRET) Image Acquisition
4:09
Determination of Crosstalk Corrections, Calibration of the Measurements, and Data Evaluation
5:13
Results: FRET Measurements in Living Plant Cells
6:22
Conclusion
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The protocol provides a simple procedure to make FRET experiments by sensitized emissions more reproducible and comparable. The main advantage of FRET are measurements in real time so that dynamic processes can be addressed. For laser adjustment u
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A protocol is provided for setting up a standard confocal laser-scanning microscope for in vivo Förster resonance energy transfer measurements, followed by data evaluation.