Sub-Mitochondrial Fractionation: Hypotonic Swelling Using Proteinase K
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Sub-Mitochondrial Fractionation: Sonication and Carbonate Extraction
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Results: Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae
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Conclusion
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This protocol is designed to determine the submitochondrial localization of yeast mitochondrial proteins, which is considered as a fundamental step during mitochondrial protein function elucidation. The protocol is suitable for our yeast strains m
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Despite recent advances, many yeast mitochondrial proteins still remain with their functions completely unknown. This protocol provides a simple and reliable method to determine the submitochondrial localization of proteins, which has been fundamental for the elucidation of their molecular functions.