Manifold Preparation and Cardiomyocyte Isolation Procedure
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Results: Isolation of High‐Quality Mouse Cardiomyocytes
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Conclusion
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This technique is more reliable and efficient and does not require extensive practice or training compared to the most commonly used Langendorff apparatus techniques. The main advantage of this technique is that it limits the ischemic time since t
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The gold standard in cardiology for cellular and molecular functional experiments are cardiomyocytes. This article describes adaptations to the non-Langendorff technique to isolate mouse cardiomyocytes.