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JoVE Journal

Cancer Research

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Subculture and Cryopreservation of Esophageal Adenocarcinoma Organoids: Pros and Cons for Single Cell Digestion

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10:42 min

July 6th, 2022

July 6th, 2022

3,615 Views

0:05

Introduction

0:53

Preparations in Advance

1:23

Harvesting Organoids

3:05

Subculturing without Digestion

4:23

Subculturing with Single-Cell Digestion

6:48

Cryopreservation of the Undigested and Single Cell Digested PDOs

8:08

Results: Observation and Identification of Cell Culture

9:46

Conclusion

Transcribir

It is an easy and visualized protocol that demonstrate handling of esophageal adenocarcinoma organoids in two different subculture process with and without single-cell digestion. The standardized protocol for subculturing EAC organoids in two diff

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This protocol describes the methods of subculture and cryopreservation of esophageal adenocarcinoma organoids with and without single cell digestion to enable researchers to choose appropriate strategies based on their experimental design.

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