April 22nd, 2022
•Here we describe the isolation, amplification, and differentiation of primary human nasal epithelial (HNE) cells at the air-liquid interface and a biobanking protocol allowing to successfully freeze and then thaw amplified HNE. The protocol analyzes electrophysiological properties of differentiated HNE cells and CFTR-related chloride secretion correction upon different modulator treatments.
Videos relacionados
Primary Human Bronchial Epithelial Cells Grown from Explants
Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
Chromatin Isolation by RNA Purification (ChIRP)
Isolation and Primary Culture of Rat Hepatic Cells
Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration
Induction and Analysis of Epithelial to Mesenchymal Transition
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice
Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados