Optical Alignment of the Combined TPEF-Stimulated Raman Scattering Microscope
4:39
Optimizing Imaging Conditions
6:36
In Vivo TPEF-SRS Imaging of Mouse Spinal Cord
8:03
Results: In Vivo Dual-Modal Imaging of Spinal Axons and Myelin Sheaths
8:31
Conclusion
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In Vivo imaging of biological tissues with subcellular resolution and chemical specificity is a powerful tool to understand the dynamic processes involved in cellular metabolism, immune response, and tissue remodeling. With combined two-photon flu
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Stimulated Raman scattering (SRS) microscopy allows label-free imaging of biomolecules based on their intrinsic vibration of specific chemical bonds. In this protocol, the instrumental setup of an integrated SRS and two-photon fluorescence microscope is described to visualize cellular structures in the spinal cord of live mice.