June 29th, 2022
•The peel-blot technique is a cryo-EM grid preparation method that allows for the separation of multilayered and concentrated biological samples into single layers to reduce thickness, increase sample concentration, and facilitate image processing.
Videos relacionados
Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Cryo-EM and Single-Particle Analysis with Scipion
A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
Single Particle Cryo-Electron Microscopy: From Sample to Structure
RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
Preparation of High-Temperature Sample Grids for Cryo-EM
Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados