Determination of the Absolute Fluorescence Intensity per Carrier
5:56
Cell Experiment
7:08
Results: A Two-Stream Methodology to Convert Relative, Semiquantitative Flow Cytometry Cell-Carrier Association Data into Absolute Quantification
8:17
Conclusion
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This protocol provides quantitative data on how carrier systems interact with cells. Quantitative data is key for engineering and optimization. It allows us to move from yes or no biological questions, to how much questions.
This technique i
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A workflow is demonstrated for the absolute quantification of drug carrier-cell interactions using flow cytometry to allow better rational evaluation of novel drug delivery systems. This workflow is applicable to drug carriers of any type.