Isolating and Extracting Extracellular Vesicles from Aortas
5:26
Assessing the Calcification Potential of Extracellular Vesicles with Light Scattering Absorbance
6:11
Assessing the Calcification Mineral Formation of Extracellular Vesicles with Collagen Hydrogels
7:39
Results: Analysis of Vascular Calcification in Collagen Hydrogels
8:23
Conclusion
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This protocol provides a method that can be used to help assess, and in the future, help develop treatments for vascular calcification, which is the most significant predictor of cardiovascular disease. The ability to isolate EVs allows us to furt
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This paper presents the methodology for obtaining and assessing vascular calcification by isolating murine aortas followed by extracting calcified extracellular vesicles to observe the mineralization potential.