Methods Article

A Procedure for Mouse Dorsal Root Ganglion Cryosectioning

5.7K Views

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04:20 min

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June 9th, 2023

DOI :

10.3791/65232-v

June 9th, 2023

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Liangliang He*¹^,², Wenxing Zhao*¹, Lingyi Zhang²^,³, Maalveka Ilango²^,⁴, Na Zhao², Liqiang Yang¹, Zhonghui Guan²

¹Department of Pain Management, Xuanwu Hospital, Capital Medical University, ²Department of Anesthesia and Perioperative Care, University of California San Francisco, ³Department of Anesthesiology, Sun Yat-Sen University, ⁴University of Washington

Transcript

Summary

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Mouse Dorsal Root Ganglion

DRG Cryosectioning

High quality Sections

Cryostat Technique

Immunochemistry Staining

RNAscope Studies

Neuropathic Pain Research

Tissue Sample Handling

Flattened Sections

OCT Sections

Protocol Development

Chapters in this video

0:00

Introduction

1:16

Dorsal Root Ganglion (DRG) Sample Preparation for Cryosectioning

3:05

Cryosectioning of the Dorsal Root Ganglion (DRG)

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