May 23rd, 2010
•Xenopus embryonic epithelia are an ideal model system to study cell behaviors such as polarity development and shape change during epithelial morphogenesis. Traditional histology of fixed samples is increasingly being complemented by live-cell confocal imaging. Here we demonstrate methods to isolate frog tissues and visualize live epithelial cells and their cytoskeleton using live-cell confocal microscopy.
Vidéos Connexes
Plastic Embedding and Sectioning of Xenopus laevis Embryos Video (Video) | JoVE
Microinjection of Xenopus Laevis Oocytes Video (Video) | JoVE
Tissue Determination Using the Animal Cap Transplant ACT Assay in Xenopus laevis Video (Video) | JoVE
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation Video (Video) | JoVE
Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast Video (Video) | JoVE
Rapid Genetic Analysis of Epithelial-Mesenchymal Signaling During Hair Regeneration Video (Video) | JoVE
Induction and Analysis of Epithelial to Mesenchymal Transition Video (Video) | JoVE
Facial Transplants in Xenopus laevis Embryos Video (Video) | JoVE
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR Video (Video) | JoVE
Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones Video (Video) | JoVE