Un merci spécial à Cori Sanders aux conceptions IROC pour la préparation des illustrations et de Kris Reddi à l&#39;UCLA pour la mise en place des cultures d&#39;échantillons de chiffres. Le financement de ce projet a été fourni par HHMI (HHMI Grant n ° 52006944).

<ol>
	<li>Barker, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> At the Bench: A Laboratory Navigator. , (1998).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Biosafety in Microbiological And Biomedical Laboratories (BMBL). , (2009).</li><li>Bykowski, T., Stevenson, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aseptic+Technique.">Aseptic Technique.</a> Current Protocols in Microbiology. Appendix 4, Appendix 4D (2008).</li><li>Cot&#233;, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aseptic+Technique+for+Cell+Culture.">Aseptic Technique for Cell Culture.</a> Current Protocols in Cell Biology. Chapter 1, Unit 1.3 (2001).</li><li>Grimes, S. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+basic+laboratory+manual+for+the+small-scale+production+and+testing+of+I-2+Newcastle+disease+vaccine.">A basic laboratory manual for the small-scale production and testing of I-2 Newcastle disease vaccine.</a> RAP Publication. AC802/E, 12-13 (2002).</li><li>Guzman, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pipetting:+A+Practical+Guide.">Pipetting: A Practical Guide.</a> The American Biology Teacher. 63 (2), 128-131 (2001).</li><li>Jordan, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> RESEARCH: National Genomics Research Initiative Phage Resource Laboratory Manual. , (2008).</li><li>Sambrook, J., Russell, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Molecular Cloning - A Laboratory Manual. , 978-087969576 (2001).</li><li>Seidman, L. A., Moore, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Basic Laboratory Methods for Biotechnology: Textbook and Laboratory Reference. , (2000).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> PIPETMAN P User's Guide. , (2012).</li></ol>

Je n&#39;ai rien à communiquer.

Une technique aseptique se réfère à une série de procédures de routine effectuées afin de prévenir des solutions stériles et des cultures de devenir contaminés par des micro-organismes indésirables dans les laboratoires. Ces techniques sont indispensables pour des expériences qui nécessitent des cellules en croissance. Bien qu&#39;un cadre de travail qui est complètement stérile ne peut être atteint, des procédures telles que la désinfection des surfaces de laboratoire, la création d&#39;un champ stérile à l&#39;aide d&#39;un bec Bunsen, en limitant l&#39;exposition des cultures non plafonnés et les médias à l&#39;air, de stérilisation de matériaux tels que des bouteilles, tubes et pipettes en verre, et en évitant tout contact avec des instruments stériles non-stériles surfaces réduit la possibilité de contamination des solutions et des cultures dans une expérience. L&#39;objectif est pour ces mesures de précaution pour devenir une seconde nature, ce qui est livré avec la formation et la pratique tout en travaillant dans un laboratoire. Transferts de volume avec des solutions stériles et des cultures en utilisant des instruments tels que pipettes sérologiques et des microropipettors sont l&#39;un des nombreux types de techniques de routine effectués dans un laboratoire. Différentes applications expérimentales appel à des instruments capables de transférer des volumes distincts, mais précis et exacts. Pipettes sérologiques sont utilisés dans les laboratoires de microbiologie pour préparer les cultures cellulaires nécessitant des préparations des médias portant sur des volumes millilitre, alors que micropipettes sont indispensables pour des expériences de biologie moléculaire qui ont besoin de montants ne microlitres de solutions. Lorsque la technique aseptique est pratiquée avec ces instruments, la contamination est réduit au minimum pendant les transferts de volume indépendamment de la quantité de liquide ou de type d&#39;expérience. Bien que n&#39;étant pas discuté dans le présent protocole, un autre moyen couramment utilisés pour prévenir la contamination est de travailler au sein d&#39;une hotte à flux laminaire (Figure 10). Cet équipement est essentiel pour la culture de tissus et pour des expériences effectuées avec des micro-organismes classés comme BLS-2 ou plus. Une hotte à flux laminaire contient un filtre HEPA (haute efficacitéparticulaire de l&#39;air) de filtre qui supprime les contaminants en suspension dans l&#39;air s&#39;écoulant le capot tout en empêchant l&#39;air non filtré de la chambre de perméation de l&#39;espace de travail. Fait à noter, un bec Bunsen ne peuvent pas être utilisés à l&#39;intérieur d&#39;une hotte à flux laminaire, car la chaleur de la flamme perturbe l&#39;écoulement de l&#39;air indispensable à la fonctionnalité de la hotte. Il est souvent utile de vérifier la qualité de votre technique aseptique lors de la réalisation d&#39;expériences. Pour valider des solutions, et des milieux de culture ne se contaminent au cours des manipulations expérimentales, toujours préparer un contrôle négatif. Par exemple, si la préparation des tubes de bouillon pour la croissance des cultures bactériennes, ne pas ensemencer un tube ne laissant que les médias stériles. Incuber le milieu aux côtés des tubes inoculés ensuite inspecter le tube témoin non inoculé des signes de contamination tels que la turbidité de la croissance de cellules indésirables involontairement introduits dans le tube. Si le tube de contrôle est contaminé, le tube d&#39;expérienceest probable sont contaminés en tant que bien, et l&#39;expérience devra être répétée. Ces mesures de précaution doit être fait avec toutes les expériences.

<table border="1"><tbody><tr><td> Nom du réactif </td><td> Entreprise </td><td> Numéro de catalogue </td><td> Commentaires </td></tr><tr><td> Bouillon LB </td><td> Difco </td><td> 244620 </td><td> Recette également disponible en référence 6 </td></tr><tr><td colspan="4"> TE Buffer: </td></tr><tr><td> Dihydraté EDTA disodique </td><td> Sigma </td><td> E5134 </td><td></td></tr><tr><td> Trizma-HCl </td><td> Sigma </td><td> T-3253 </td><td></td></tr><tr><td> CiDecon </td><td> Decon Laboratories, Inc </td><td> 8504 </td><td> Désinfectant </td></tr><tr><td> L&#39;éthanol </td><td> Fisher Scientific </td><td> A406 </td><td> Pour utilisation comme désinfectant, préparer 70% (v / v) avec de l&#39;eau distillée </td></tr></tbody></table>

aseptic laboratory techniques: volume transfers with serological pipettes and micropipettors

Micro-organismes sont partout - dans l&#39;air, le sol, et le corps humain ainsi que sur des surfaces inanimées comme des bancs de laboratoire et les claviers d&#39;ordinateur. L&#39;omniprésence des microbes crée une offre abondante des contaminants potentiels dans un laboratoire. Pour assurer le succès expérimental, le nombre de contaminants sur les surfaces de l&#39;équipement et les travaux doivent être minimisés. Commune parmi les nombreuses expériences en microbiologie sont des techniques associées à la mesure et le transfert des cultures contenant des cellules bactériennes ou des particules virales. Pour ce faire, sans contact avec les surfaces non stériles ou de contaminer les médias stériles exige (1) préparer un espace de travail stérile, (2) à un réglage précis et avec précision la lecture des instruments pour le transfert aseptique des liquides, et (3) correctement la manipulation des instruments, des flacons, des bouteilles et des cultures des tubes à l&#39;intérieur d&#39;un champ stérile. L&#39;apprentissage de ces procédures exige une formation et la pratique. Dans un premier temps, les actions devraient être lent, délibéré et contrôlé par l&#39;objectif étant pour t aseptiquesechnique pour devenir une seconde nature lorsque l&#39;on travaille sur le banc. Nous présentons ici les étapes à suivre pour mesurer les volumes utilisant des pipettes sérologiques et Micropipettes dans un champ stérile créée par un bec Bunsen. Plage de volumes de microlitres (ul) en millilitres (ml) en fonction de l&#39;instrument utilisé. Liquides couramment transférés comprennent bouillon stérile ou des solutions chimiques, ainsi que des cultures bactériennes et des stocks de phages. En suivant ces procédures, les élèves devraient être en mesure de: <ul><li> Le travail au sein du champ stérile créée par la flamme du brûleur Bunsen. </li><li> N&#39;utiliser que des pipettes sérologiques sans compromettre la stérilité instrument. </li><li> Aspirer des liquides avec des pipettes sérologiques, précisément la lecture des volumes calibrés en alignant le ménisque formé par le liquide à des marques de graduation de la pipette. </li><li> Conservez les bouteilles, flacons, culture tubes et leurs casquettes respectives stériles lors des transferts de liquides. </li><li> Identifier les différentes applications pour le plastique contre verre sérologique pipettes. </li><li> Limites de précision d&#39;État pour micropipettes. </li><li> Précisément, et régler avec précision des volumes sur micropipettes. </li><li> Savoir comment utiliser correctement l&#39;arrêt de première et deuxième sur une micropipette pour aspirer et transférer des volumes corrects. </li></ul>

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Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors

Lorsque vous travaillez dans un laboratoire, il est impératif de minimiser les sources de contamination. Une technique aseptique se réfère aux procédures qui permettent le transfert des cultures et des réactifs, tout en évitant le contact avec les surfaces non stériles. Pipettes sérologiques et Micropipettes sont utilisés pour mesurer des volumes précis sans compromettre la stérilité des solutions utilisées dans les expériences.

Techniques de laboratoire aseptiques: Transferts en volume avec pipettes sérologiques et Micropipettes

Microorganisms are everywhere - in the air, soil, and human body as well as on inanimate surfaces like laboratory benches and computer keyboards. The ...

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161.8K vues.  University of California, Los Angeles. Lorsque vous travaillez dans un laboratoire, il est impératif de minimiser les sources de contamination. Une technique aseptique se réfère aux procédures qui permettent le transfert des cultures et des réactifs, tout en évitant le contact avec les surfaces non stériles. Pipettes sérologiques et Micropipettes sont utilisés pour mesurer des volumes précis sans compromettre la stérilité des solutions utilisées dans les expériences.

Vidéo: Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors

Microbial Communities in Nature and Laboratory - Interview

Les communautés microbiennes dans la Nature et de laboratoire - Interview

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Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Glass microelectrodes (also called pipettes) have been a workhorse of electrophysiology for decades. Today, such pipettes are made from glass capillaries using a programmable puller. Such instruments heat the capillary using either a metal filament or a laser and draw out the glass using gravity, a motor or both. Pipettes for patch-clamp recording are formed using only heat and gravity, while sharp electrodes for intracellular recording use a combination of heat, gravity, and a motor. The procedure used to make intracellular recording pipettes is similar to that used to make injection needles for a variety of applications, including cRNA injection into Xenopus oocytes. In general, capillary glass &lt;1.2 mm in diameter is used to make pipettes for patch clamp recording, while narrower glass is used for intracellular recording (outer diameter = 1.0 mm). For each tool, the puller is programmed slightly differently. This video shows how to make both kinds of recording pipettes using pre-established puller programs.

This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.

Faire patch pipettes et électrodes Sharp avec un extracteur programmable

Glass microelectrodes (also called pipettes) have been a workhorse of electrophysiology for decades.  Today, such pipettes are made from glass capillaries ...

Pressure-polishing Pipettes for Improved Patch-clamp Recording

Pressure-polishing is a method for shaping glass pipettes for patch-clamp recording. We first developed this method for fabricating pipettes suitable for recording from small (&lt;3 m) neuronal cell bodies. The basic principal is similar to glass-blowing and combines air pressure and heat to modify the shape of patch pipettes prepared by a conventional micropipette puller. It can be applied to so-called soft (soda lime) and hard (borosilicate) glasses. Generally speaking, pressure polishing can reduce pipette resistance by 25% without decreasing the diameter of the tip opening (Goodman and Lockery, 2000). It can be applied to virtually any type of glass and requires only the addition of a high-pressure valve and fitting to a microforge. This technique is essential for recording from ultrasmall cells (&lt;5 m) and can also improve single-channel recording by minimizing pipette resistance. The blunt shape is also useful for perforated-patch clamp recording since this tip shape results in a larger membrane bleb available for perforation.

This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.

Pression de polissage Pipettes Amélioration patch-clamp

Pressure-polishing is a method for shaping glass pipettes for patch-clamp recording.  We first developed this method for fabricating pipettes suitable for ...

Modeling Biological Membranes with Circuit Boards and Measuring Electrical Signals in Axons: Student Laboratory Exercises

This is a demonstration of how electrical models can be used to characterize biological membranes. This exercise also introduces biophysical terminology used in electrophysiology. The same equipment is used in the membrane model as on live preparations. Some properties of an isolated nerve cord are investigated: nerve action potentials, recruitment of neurons, and responsiveness of the nerve cord to environmental factors.

This is a demonstration of how biological membranes can be understood using electrical models. We also demonstrate procedures for recording action potentials from the ventral nerve cord of the crayfish for student orientated laboratories.

Modélisation des membranes biologiques avec des cartes de circuits et la mesure des signaux électriques dans les axones: Exercices de laboratoire étudiant

This is a demonstration of how electrical models can be used to characterize biological membranes. This exercise also introduces biophysical terminology ...

Aseptic Laboratory Techniques: Plating Methods

Microorganisms are present on all inanimate surfaces creating ubiquitous sources of possible contamination in the laboratory. Experimental success relies on the ability of a scientist to sterilize work surfaces and equipment as well as prevent contact of sterile instruments and solutions with non-sterile surfaces. Here we present the steps for several plating methods routinely used in the laboratory to isolate, propagate, or enumerate microorganisms such as bacteria and phage. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials. Procedures described include (1) streak-plating bacterial cultures to isolate single colonies, (2) pour-plating and (3) spread-plating to enumerate viable bacterial colonies, (4) soft agar overlays to isolate phage and enumerate plaques, and (5) replica-plating to transfer cells from one plate to another in an identical spatial pattern. These procedures can be performed at the laboratory bench, provided they involve non-pathogenic strains of microorganisms (Biosafety Level 1, BSL-1). If working with BSL-2 organisms, then these manipulations must take place in a biosafety cabinet. Consult the most current edition of the Biosafety in Microbiological and Biomedical Laboratories (BMBL) as well as Material Safety Data Sheets (MSDS) for Infectious Substances to determine the biohazard classification as well as the safety precautions and containment facilities required for the microorganism in question. Bacterial strains and phage stocks can be obtained from research investigators, companies, and collections maintained by particular organizations such as the American Type Culture Collection (ATCC). It is recommended that non-pathogenic strains be used when learning the various plating methods. By following the procedures described in this protocol, students should be able to: 
 
&#9679; Perform plating procedures without contaminating media. 
&#9679; Isolate single bacterial colonies by the streak-plating method. 
&#9679; Use pour-plating and spread-plating methods to determine the concentration of bacteria. 
&#9679; Perform soft agar overlays when working with phage. 
&#9679; Transfer bacterial cells from one plate to another using the replica-plating procedure. 
&#9679; Given an experimental task, select the appropriate plating method.

When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.

Techniques de laboratoire aseptiques: Méthodes de placage

Microorganisms are present on all inanimate surfaces creating ubiquitous sources of possible contamination in the laboratory. Experimental success relies ...

Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands

Histology volume reconstruction facilitates the study of 3D shape and volume change of an organ at the level of macrostructures made up of cells. It can also be used to investigate and validate novel techniques and algorithms in volumetric medical imaging and therapies. Creating 3D high-resolution atlases of different organs1,2,3 is another application of histology volume reconstruction. This provides a resource for investigating tissue structures and the spatial relationship between various cellular features. We present an image registration approach for histology volume reconstruction, which uses a set of optical blockface images. The reconstructed histology volume represents a reliable shape of the processed specimen with no propagated post-processing registration error. The Hematoxylin and Eosin (H&#38;E) stained sections of two mouse mammary glands were registered to their corresponding blockface images using boundary points extracted from the edges of the specimen in histology and blockface images. The accuracy of the registration was visually evaluated. The alignment of the macrostructures of the mammary glands was also visually assessed at high resolution.
This study delineates the different steps of this image registration pipeline, ranging from excision of the mammary gland through to 3D histology volume reconstruction. While 2D histology images reveal the structural differences between pairs of sections, 3D histology volume provides the ability to visualize the differences in shape and volume of the mammary glands.

We present an image registration approach for 3-dimensional (3D) histology volume reconstruction, which facilitates the study of the changes of an organ at the level of macrostructures made up of cells . Using this approach, we studied the 3D changes between wild-type and Igfbp7-null mammary glands.

Reconstruction de 3-Dimensional Volume histologique et son application à l&#39;étude de souris glandes mammaires

Histology volume reconstruction facilitates the study of 3D shape and volume change of an organ at the level of macrostructures made up of cells. It can ...

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Extracellular Vesicles (EVs) are small, membrane-derived vesicles found in bodily fluids that are highly involved in cell-cell communication and help regulate a diverse range of biological processes. Analysis of EVs using flow cytometry (FCM) has been notoriously difficult due to their small size and lack of discrete populations positive for markers of interest. Methods for EV analysis, while considerably improved over the last decade, are still a work in progress. Unfortunately, there is no one-size-fits-all protocol, and several aspects must be considered when determining the most appropriate method to use. Presented here are several different techniques for processing EVs and two protocols for analyzing EVs using either individual detection or a bead-based approach. The methods described here will assist with eliminating the antibody aggregates commonly found in commercial preparations, increasing signal&#8211;to-noise ratio, and setting gates in a rational fashion that minimizes detection of background fluorescence. The first protocol uses an individual detection method that is especially well suited for analyzing a high volume of clinical samples, while the second protocol uses a bead-based approach to capture and detect smaller EVs and exosomes.

Many different methods exist for the measurement of extracellular vesicles (EVs) using flow cytometry (FCM). Several aspects should be considered when determining the most appropriate method to use. Two protocols for measuring EVs are presented, using either individual detection or a bead-based approach.

Techniques pour l&#39;analyse des vésicules extracellulaire par cytométrie en flux

Extracellular Vesicles (EVs) are small, membrane-derived vesicles found in bodily fluids that are highly involved in cell-cell communication and help ...

Measuring Pressure Volume Loops in the Mouse

Understanding the causes and progression of heart disease presents a significant challenge to the biomedical community. The genetic flexibility of the mouse provides great potential to explore cardiac function at the molecular level. The mouse&#39;s small size does present some challenges in regards to performing detailed cardiac phenotyping. Miniaturization and other advancements in technology have made many methods of cardiac assessment possible in the mouse. Of these, the simultaneous collection of pressure and volume data provides a detailed picture of cardiac function that is not available through any other modality. Here a detailed procedure for the collection of pressure-volume loop data is described. Included is a discussion of the principles underlying the measurements and the potential sources of error. Anesthetic management and surgical approaches are discussed in great detail as they are both critical to obtaining high quality hemodynamic measurements. The principles of hemodynamic protocol development and relevant aspects of data analysis are also addressed.

This manuscript describes a detailed protocol for the collection of pressure-volume data from the mouse.

Mesure de boucles de volume de pression chez la souris

Understanding the causes and progression of heart disease presents a significant challenge to the biomedical community. The genetic flexibility of the ...

Techniques to Induce and Quantify Cellular Senescence

In response to cellular stress or damage, proliferating cells can induce a specific program that initiates a state of long-term cell-cycle arrest, termed cellular senescence. Accumulation of senescent cells occurs with organismal aging and through continual culturing in vitro. Senescent cells influence many biological processes, including embryonic development, tissue repair and regeneration, tumor suppression, and aging. Hallmarks of senescent cells include, but are not limited to, increased senescence-associated &#946;-galactosidase activity (SA-&#946;-gal); p16INK4A, p53, and p21 levels; higher levels of DNA damage, including &#947;-H2AX; the formation of Senescence-associated Heterochromatin Foci (SAHF); and the acquisition of a Senescence-associated Secretory Phenotype (SASP), a phenomenon characterized by the secretion of a number of pro-inflammatory cytokines and signaling molecules. Here, we describe protocols for both replicative and DNA damage-induced senescence in cultured cells. In addition, we highlight techniques to monitor the senescent phenotype using several senescence-associated markers, including SA-&#946;-gal, &#947;-H2AX and SAHF staining, and to quantify protein and mRNA levels of cell cycle regulators and SASP factors. These methods can be applied to the assessment of senescence in various models and tissues.

Cellular senescence, the irreversible state of cell-cycle arrest, can be induced by various cellular stresses. Here, we describe protocols to induce cellular senescence and methods to assess markers of senescence.

Techniques pour induire et Quantifier cellulaire sénescence

In response to cellular stress or damage, proliferating cells can induce a specific program that initiates a state of long-term cell-cycle arrest, termed ...

Volume Segmentation and Analysis of Biological Materials Using SuRVoS (Super-region Volume Segmentation) Workbench

Segmentation is the process of isolating specific regions or objects within an imaged volume, so that further study can be undertaken on these areas of interest. When considering the analysis of complex biological systems, the segmentation of three-dimensional image data is a time consuming and labor intensive step. With the increased availability of many imaging modalities and with automated data collection schemes, this poses an increased challenge for the modern experimental biologist to move from data to knowledge. This publication describes the use of SuRVoS Workbench, a program designed to address these issues by providing methods to semi-automatically segment complex biological volumetric data. Three datasets of differing magnification and imaging modalities are presented here, each highlighting different strategies of segmenting with SuRVoS. Phase contrast X-ray tomography (microCT) of the fruiting body of a plant is used to demonstrate segmentation using model training, cryo electron tomography (cryoET) of human platelets is used to demonstrate segmentation using super- and megavoxels, and cryo soft X-ray tomography (cryoSXT) of a mammalian cell line is used to demonstrate the label splitting tools. Strategies and parameters for each datatype are also presented. By blending a selection of semi-automatic processes into a single interactive tool, SuRVoS provides several benefits. Overall time to segment volumetric data is reduced by a factor of five when compared to manual segmentation, a mainstay in many image processing fields. This is a significant savings when full manual segmentation can take weeks of effort. Additionally, subjectivity is addressed through the use of computationally identified boundaries, and splitting complex collections of objects by their calculated properties rather than on a case-by-case basis.

Volume Segmentation and Analysis of Biological Materials Using SuRVoS Super-region Volume Segmentation Workbench

Segmentation of three-dimensional data from many imaging techniques is a major bottleneck in analysis of complex biological systems. Here, we describe the use of SuRVoS Workbench to semi-automatically segment volumetric data at various length-scales using example datasets from cryo-electron tomography, cryo soft X-ray tomography, and phase contrast X-ray tomography techniques.

Volume de Segmentation et analyse des matériaux biologiques à l’aide de Workbench SuRVoS (Super-région Volume Segmentation)

Segmentation is the process of isolating specific regions or objects within an imaged volume, so that further study can be undertaken on these areas of ...