May 5th, 2014
•In vivo transfection of naked DNA by hydrodynamic gene delivery introduces genes into the tissue of an animal with minimal inflammatory response. Sufficient amounts of gene product are generated such that gene function and regulation as well as protein structure and function can be analyzed.
Vidéos Connexes
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid Video (Video) | JoVE
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency Video (Video) | JoVE
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells Video (Video) | JoVE
Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles Video (Video) | JoVE
In vitro Reconstitution of the Active T. castaneum Telomerase Video (Video) | JoVE
Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins Video (Video) | JoVE
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia Video (Video) | JoVE
SiRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells Video (Video) | JoVE
Measurement of Metabolic Rate in Drosophila using Respirometry Video (Video) | JoVE
High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension Video (Video) | JoVE