This work was supported by the Deutsche Forschungsgemeinschaft (DU 1190/3-1).

Toutes les expériences sur les animaux ont été effectuées en conformité avec la loi de protection des animaux allemand (TierSchG). Les souris ont été logées et manipulés conformément aux bonnes pratiques de l&#39;animal tel que défini par la FELASA (www.felasa.eu/guidelines.php) et le corps de bien-être animal nationale GV-SOLAS (www.gv-solas.de/index.html). Le comité de protection des animaux de l&#39;Université de Fribourg ainsi que les autorités locales (Regierungspräsidium Freiburg) ont approuvé toutes les expérimentations animales. 1. manipulation des animaux, préparation et induction de l&#39;inflammation <ol><li> Utilisez 4-6 semaine Enfant souris mâles avec une gamme de poids de 16 - 20 g. Remarque: Si les souris sont plus âgés ou plus lourd qu&#39;ils présentent graisse excessive entourant la cuve, limitant l&#39;observation microscopique. </li><li> Stériliser tous les outils et table de microscope avant la chirurgie. </li><li> Injecter 20 mg / kg de LPS (lipopolysaccharide) par voie intraperitoneale quatre heures avant la microscopie dans la vie de souris pour induire une Inflamm bactérienneation. </li><li> Préchauffer une solution saline à 0,9% dans un bain-marie à 37 ° C pour humidifier la chambre en matière plastique et le tissu mésentère. </li><li> Anesthésier les souris avec une injection intraperitoneale de kétamine (100 mg / kg) et de xylazine (5 mg / kg) juste avant que la procédure de microscopie. Anesthésie alternatifs peuvent être utilisés, par exemple, 2% inhalation d&#39;isoflurane. Confirmez anesthésie appropriée par la perte de la réponse à la stimulation réflexe (orteil ou pincement de la queue avec une pression ferme). </li><li> Épiler l&#39;abdomen à l&#39;aide d&#39;un rasoir et enlever les poils lâche avec de la gaze saturé avec de l&#39;éthanol 70%. </li><li> Appliquer vétérinaire pommade sur les yeux de la souris pour prévenir la sécheresse, tandis que sous anesthésie. </li></ol> 2. Chirurgie <ol><li> Stériliser l&#39;abdomen en utilisant 70% d&#39;éthanol. Cette méthode est une méthode non stérile et peut être mortelle à la fin de l&#39;expérience. </li><li> Effectuez une laparotomie médiane: Ouvrez la peau de l&#39;abdomen à l&#39;aide de petites pinces courbes et de petits ciseaux. Identifier l&#39;épigastrical navires et ouvrir le péritoine dans la région de la ligne blanche, à protéger les vaisseaux. </li><li> Appliquez quelques gouttes de sérum physiologique préchauffé dans la cavité abdominale de garder le tissu humide. </li><li> des leucocytes et des plaquettes d&#39;étiquetage par fluorescence en injectant 50 ul rhodamine 6G (1 mg / ml) rétro-orbitale, comme décrit avant 9,10. </li><li> Extérioriser une boucle de ileumand lieu dans une boîte de Petri d&#39;un diamètre de 10 cm et assurez-vous de garder le tissu humide en appliquant la 37 ° C préchauffé solution saline (0,9%) tous les autres minutes. </li></ol> 3. Microscopie intravitale <ol><li> Passer la souris sous le microscope et amener la veine mésentérique d&#39;un diamètre de 200 à 300 um dans le centre de la vue. Choisissez un navire sans environnement gras visible. </li><li> Ne pas toucher les vaisseaux mesentery évitant ainsi la stimulation de l&#39;endothélium. Manipulez la boucle de l&#39;iléon prudence. </li><li> Visualiser les interactions entre cellules endothéliales sanguinsavec un microscope inversé ou verticale et une caméra à l&#39;aide d&#39;un logiciel d&#39;microscope. Fiche sang des interactions des cellules endothéliales pendant 1 min à 4 veines différentes par souris. </li><li> Euthanasier la souris par dislocation cervicale après l&#39;achèvement d&#39;expériences d&#39;imagerie. </li></ol> 4. Analyse <ol><li> Effectuer l&#39;analyse hors ligne et aveuglé pour tous les paramètres. Procéder à une analyse en utilisant n&#39;importe quel logiciel approprié. </li><li> Confirmez conditions stables et comparables interindividually flux sanguin en haute résolution temporelle ciné-clips (taux de trame maximale) ont porté sur les flux de globules intraluminal. </li><li> Quantifier le nombre de leucocytes roulement. Avoisinant tracez une ligne verticale à travers la veine et compter tous les leucocytes qui traversent cette ligne en 1 min. </li><li> Déterminer la vitesse de roulement en mesurant le temps d&#39;une seule leucocytes a besoin de passer à une distance de 50 um tout en roulant de manière stable sur l&#39;endothélium. Pour ce faire, tirer deux lignes verticales sur une distance de 50 um trâce la veine. <ol><li> Mesurer le temps un leucocyte représentant a besoin d&#39;obtenir d&#39;une ligne à l&#39;other.Calculate la vitesse des leucocytes en divisant 50 um à travers le temps nécessaire (um / s). </li></ol></li><li> Mesurer l&#39;adhérence des leucocytes dans un domaine de 0,04 mm 2. Pour ce faire, dessiner un carré avec la longueur de côté de 200 um dans la veine. <ol><li> Comptez le nombre de leucocytes adhérents fermes, définis comme aucun mouvement visible pendant 30 secondes, dans ce carré. </li></ol></li><li> Comptez le nombre de plaquettes liés à l&#39;un des leucocytes à quantifier les interactions plaquettes-leucocytes. </li></ol>

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V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+investigations+of+the+adhesiveness+of+circulating+polymorphonuclear+leucocytes+to+blood+vessel+walls.">Quantitative investigations of the adhesiveness of circulating polymorphonuclear leucocytes to blood vessel walls.</a> The Journal of physiology. 222, 447-474 (1972).</li><li>Duerschmied, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Serotonin+stimulates+platelet+receptor+shedding+by+tumor+necrosis+factor-alpha-converting+enzyme+(ADAM17).">Serotonin stimulates platelet receptor shedding by tumor necrosis factor-alpha-converting enzyme (ADAM17).</a> Journal of thrombosis and haemostasis : JTH. 7, 1163-1171 (2009).</li><li>Chauhan, A. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ADAMTS13:+a+new+link+between+thrombosis+and+inflammation.">ADAMTS13: a new link between thrombosis and inflammation.</a> The Journal of experimental medicine. 205, 2065-2074 (1084).</li><li>Baez, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+open+cremaster+muscle+preparation+for+the+study+of+blood+vessels+by+in+vivo+microscopy.">An open cremaster muscle preparation for the study of blood vessels by in vivo microscopy.</a> Microvascular research. 5, 384-394 (1973).</li><li>Leister, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+peritoneal+cavity+chamber+for+intravital+microscopy+of+the+liver+under+conditions+of+pneumoperitoneum.">A peritoneal cavity chamber for intravital microscopy of the liver under conditions of pneumoperitoneum.</a> Surgical endoscopy. 17, 939-942 (2003).</li><li>Duerschmied, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Platelet+serotonin+promotes+the+recruitment+of+neutrophils+to+sites+of+acute+inflammation+in+mice.">Platelet serotonin promotes the recruitment of neutrophils to sites of acute inflammation in mice.</a> Blood. 121, 1008-1015 (2013).</li><li>Herr, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acute+fluoxetine+treatment+induces+slow+rolling+of+leukocytes+on+endothelium+in+mice.">Acute fluoxetine treatment induces slow rolling of leukocytes on endothelium in mice.</a> PloS one. 9, e88316 (2014).</li><li>Yardeni, T., Eckhaus, M., Morris, H. 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The authors have nothing to disclose.

Nous décrivons ici la préparation et la performance de la microscopie intravitale de veines de mésentère des souris in vivo. Cette méthode nous donne l&#39;occasion d&#39;observer les interactions leucocytes-endothélial et des plaquettes-endothélial 11 directement dans l&#39;organisme vivant. Comme un début de réponse à l&#39;inflammation de l&#39;endothélium obtient activé et interagit avec les leucocytes et les plaquettes entraînant dans le laminage, l&#39;...

Le but de ce protocole est de décrire une technique simplifiée de microscopie intravitale de veines de mesentery chez les souris vivant pour l&#39;observation directe des interactions leucocytes-endothélial et leuco-plaquettaire dans des conditions inflammatoires. Microscopie intravitale a été développé pour étudier les interactions leucocytes-endothélial in vivo dans des conditions inflammatoires 1,2, ce qui est non pas anodine mais importante pour comprendre le recrutement des leucocytes inflammatoires et la fonction. La méthode que nous décrivons dans ce protocole a été développé sur la base de publications publiées antérieurement. Semblable à la visualisation plaquettes incorporation dans un thrombus croissante 3 et parallèlement à ce qui a été publié plus tôt 4, une veine mésentérique extériorisé est examiné par microscopie lumière transmise. Il existe divers autres modèles de la microscopie intra-vital tels que le muscle crémaster de rats 5 ou le foie des souris et des rats 6. Microscopie intravitale du mésentère veine a été développée et appliquée dans les études précédentes par plusieurs groupes. Nous avons utilisé cette technique pour observer des différences dans le recrutement des leucocytes entre les souris de type sauvage et le tryptophane hydroxylase1 (TPH1) - / - souris, qui sont déficientes de la sérotonine non-neuronales et comparé les résultats à des souris dont la sérotonine plaquettaire a été pharmacologiquement appauvri par à long terme inhibiteur de la demande d&#39;un recaptage de la sérotonine fluoxétine 7. Nous avons également examiné les interactions leucocytes-endothélial après le traitement par la fluoxétine aiguë 8. Dans ce protocole, nous nous concentrons sur la microscopie intravitale de veines de mesentery, parce que ce modèle peut être réalisé rapidement, et permet des mesures valides de interactions leucocytes-endothélial et des plaquettes-leucocytes. Cela est beaucoup plus difficile et beaucoup de temps dans la microscopie intravitale d&#39;autres organes, tels que les os, le foie, la peau, ou le muscle crémaster. Le model décrite ici est idéale pour l&#39;évaluation reproductible inflammatoire de l&#39;interaction cellule-cellule avec challengeing après un stimulus inflammatoire, comme l&#39;injection intraperitoneale de lipopolysaccharide.

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>Rhodamine 6G</td>
			<td>Sigma-Aldrich</td>
			<td>&#160;989-38-8</td>
			<td></td>
		</tr>
		<tr>
			<td>Lipopolysaccharides from Escherichia coli 055:B5</td>
			<td>Sigma-Aldrich</td>
			<td>L2637&#160;</td>
			<td></td>
		</tr>
	</tbody>
</table>

intravital microscopy of leukocyte-endothelial and platelet-leukocyte interactions in mesenterial veins in mice

Microscopie intravitale est une méthode qui peut être utilisée pour étudier des processus différents dans les différentes régions et des vaisseaux chez les animaux vivants. Dans ce protocole, nous décrivons la microscopie intravitale de veines de mesentery. Ceci peut être réalisé en un court laps de temps avec des résultats reproductibles montrant les interactions des leucocytes-endothélial in vivo. Nous décrivons un cadre inflammatoire après LPS défi de l&#39;endothélium. Mais dans ce modèle on peut appliquer de nombreux types différents de conditions inflammatoires, comme bactérienne, chimique ou biologique et d&#39;enquêter sur l&#39;administration de médicaments et de leurs effets directs sur l&#39;animal vivant et son impact sur le recrutement des leucocytes. Ce protocole a été appliqué avec succès à un certain nombre de traitements différents de souris et de leurs effets sur la réponse inflammatoire dans les vaisseaux. Ici, nous décrivons la visualisation des leucocytes et des plaquettes par marquage par fluorescence de la rhodamine 6G avec ceux-ci. En outre, toute imagerie spécifique peut être performed en utilisant des molécules cibles marquées par fluorescence.

Un aperçu de la création expérimentale décrite dans ce protocole est montré à la figure 1. Il montre une souris avec un iléon boucle extériorisée et ses navires, qui peut être observé en microscopie intravitale. La figure 2 montre certain degré d&#39;activation chez les animaux non traités en raison de la procédure elle-même. Mais il n&#39;y a presque pas de roulement lent ou adhésion ferme des leucocytes par rapport à des souris LPS-traités. La figure 2</stron...

Watch this Scientific Journal Video about Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice at JoVE.com

Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice

This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.

Interactions Microscopie intravitale des leucocytes endothéliaux et plaquettes-leucocytes dans mésentériques veines chez les souris

Intravital microscopy is a method that can be used to investigate different processes in different regions and vessels in living animals. In this ...

intravital-microscopy-leukocyte-endothelial-platelet-leukocyte

Research

JoVE Journal

Immunology and Infection

12.0K vues.  Heart Center, University of Freiburg. This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.

Vidéo: Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice

Intravital Microscopy of the Inguinal Lymph Node

Lymph nodes (LN's), located throughout the body, are an integral component of the immune system. They serve as a site for induction of adaptive immune response and therefore, the development of effector cells. As such, LNs are key to fighting invading pathogens and maintaining health. The choice of LN to study is dictated by accessibility and the desired model; the inguinal lymph node is well situated and easily supports studies of biologically relevant models of skin and genital mucosal infection.
The inguinal LN, like all LNs, has an extensive microvascular network supplying it with blood. In general, this microvascular network includes the main feed arteriole of the LN that subsequently branches and feeds high endothelial venules (HEVs). HEVs are specialized for facilitating the trafficking of immune cells into the LN during both homeostasis and infection. How HEVs regulate trafficking into the LN under both of these circumstances is an area of intense exploration. The LN feed arteriole, has direct upstream influence on the HEVs and is the main supply of nutrients and cell rich blood into the LN. Furthermore, changes in the feed arteriole are implicated in facilitating induction of adaptive immune response. The LN microvasculature has obvious importance in maintaining an optimal blood supply to the LN and regulating immune cell influx into the LN, which are crucial elements in proper LN function and subsequently immune response. 
The ability to study the LN microvasculature in vivo is key to elucidating how the immune system and the microvasculature interact and influence one another within the LN. Here, we present a method for in vivo imaging of the inguinal lymph node. We focus on imaging of the microvasculature of the LN, paying particular attention to methods that ensure the study of healthy vessels, the ability to maintain imaging of viable vessels over a number of hours, and quantification of vessel magnitude. Methods for perfusion of the microvasculature with vasoactive drugs as well as the potential to trace and quantify cellular traffic are also presented. 
Intravital microscopy of the inguinal LN allows direct evaluation of microvascular functionality and real-time interface of the direct interface between immune cells, the LN, and the microcirculation. This technique potential to be combined with many immunological techniques and fluorescent cell labelling as well as manipulated to study vasculature of other LNs.

A technique for performing intravital microscopy of the inguinal lymph node (LN) is outlined. Such technique allows for real-time, in vivo study of the lymph node microvasculature and structure both during homeostasis and infection. This technique can be adapted to cell trafficking studies and to other lymph node sites.

Microscopie intravitale du ganglion lymphatique inguinal

Lymph nodes (LN's), located throughout the body, are an integral component of the immune system. They serve as a site for induction of adaptive immune ...

Recherche

Immunologie et infection

Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy

The recruitment of circulating leukocytes from blood stream to the inflamed tissue is a crucial and complex process of inflammation1,2. In the postcapillary venules of inflamed tissue, leukocytes initially tether and roll on the luminal surface of venular wall. Rolling leukocytes arrest on endothelium and undergo firm adhesion in response to chemokine or other chemoattractants on the venular surface. Many adherent leukocytes relocate from the initial site of adhesion to the junctional extravasation site in endothelium, a process termed intraluminal crawling3. Following crawling, leukocytes move across endothelium (transmigration) and migrate in extravascular tissue toward the source of chemoattractant (chemotaxis)4. Intravital microscopy is a powerful tool for visualizing leukocyte-endothelial cell interactions in vivo and revealing cellular and molecular mechanisms of leukocyte recruitment2,5. In this report, we provide a comprehensive description of using brightfield intravital microscopy to visualize and determine the detailed processes of neutrophil recruitment in mouse cremaster muscle in response to the gradient of a neutrophil chemoattractant. To induce neutrophil recruitment, a small piece of agarose gel (~1-mm3 size) containing neutrophil chemoattractant MIP-2 (CXCL2, a CXC chemokine) or WKYMVm (Trp-Lys-Tyr-Val-D-Met, a synthetic analog of bacterial peptide) is placed on the muscle tissue adjacent to the observed postcapillary venule. With time-lapsed video photography and computer software ImageJ, neutrophil intraluminal crawling on endothelium, neutrophil transendothelial migration and the migration and chemotaxis in tissue are visualized and tracked. This protocol allows reliable and quantitative analysis of many neutrophil recruitment parameters such as intraluminal crawling velocity, transmigration time, detachment time, migration velocity, chemotaxis velocity and chemotaxis index in tissue. We demonstrate that using this protocol, these neutrophil recruitment parameters can be stably determined and the single cell locomotion conveniently tracked in vivo.

We describe a protocol of brightfield intravital microscopy for measuring dynamic neutrophil-endothelial cell interactions during neutrophil recruitment in response to the source of a neutrophil chemoattractant in vivo. Neutrophil intraluminal crawling, transendothelial migration and chemotaxis in mouse cremaster muscle tissue are visualized with time-lapsed video photography and tracked with ImageJ.

Suivi des rampants neutrophiles intraluminale, migration trans et le chimiotactisme dans les tissus par microscopie intravitale Vidéo

The recruitment of circulating leukocytes from blood stream to the inflamed tissue is a crucial and complex process of inflammation1,2. In the ...

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Two-photon Microscopy (TPM) provides image acquisition in deep areas inside tissues and organs. In combination with the development of new stereotactic tools and surgical procedures, TPM becomes a powerful technique to identify "niches" inside organs and to document cellular "behaviors" in live animals. While intravital imaging provides information that best resembles the real cellular behavior inside the organ, it is both more laborious and technically demanding in terms of required equipment/procedures than alternative ex vivo imaging acquisition. Thus, we describe a surgical procedure and novel "stereotactic" organ holder that allows us to follow the movements of Foxp3+ cells within the thymus.
Foxp3 is the master regulator for the generation of regulatory T cells (Tregs). Moreover, these cells can be classified according to their origin: ie. thymus-differentiated Tregs are called "naturally-occurring Tregs" (nTregs), as opposed to peripherally-converted Tregs (pTregs). Although significant amount of research has been reported in the literature concerning the phenotype and physiology of these T cells, very little is known about their in vivo interactions with other cells. This deficiency may be due to the absence of techniques that would permit such observations. The protocol described in this paper provides a remedy for this situation.
Our protocol consists of using nude mice that lack an endogenous thymus since they have a punctual mutation in the DNA sequence that compromises the differentiation of some epithelial cells, including thymic epithelial cells. Nude mice were gamma-irradiated and reconstituted with bone marrows (BM) from Foxp3-KIgfp/gfp mice. After BM recovery (6 weeks), each animal received embryonic thymus transplantation inside the kidney capsule. After thymus acceptance (6 weeks), the animals were anesthetized; the kidney containing the transplanted thymus was exposed, fixed in our organ holder, and kept under physiological conditions for in vivo imaging by TPM. We have been using this approach to study the influence of drugs in the generation of regulatory T cells.

We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of &#x201C;niches&#x201D; within the thymus where T cell development occurs.

Imagerie intravitale du Thymus souris utilisant la 2-microscopie biphotonique

Two-photon Microscopy (TPM) provides image acquisition in deep areas inside tissues and organs. In combination with the development of new stereotactic ...

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

The advent of intravital microscopy in experimental rodent malaria models has allowed major advances to the knowledge of parasite-host interactions 1,2. Thus, in vivo imaging of malaria parasites during pre-erythrocytic stages have revealed the active entrance of parasites into skin lymph nodes 3, the complete development of the parasite in the skin 4, and the formation of a hepatocyte-derived merosome to assure migration and release of merozoites into the blood stream 5. Moreover, the development of individual parasites in erythrocytes has been recently documented using 4D imaging and challenged our current view on protein export in malaria 6. Thus, intravital imaging has radically changed our view on key events in Plasmodium development. Unfortunately, studies of the dynamic passage of malaria parasites through the spleen, a major lymphoid organ exquisitely adapted to clear infected red blood cells are lacking due to technical constraints.
Using the murine model of malaria Plasmodium yoelii in Balb/c mice, we have implemented intravital imaging of the spleen and reported a differential remodeling of it and adherence of parasitized red blood cells (pRBCs) to barrier cells of fibroblastic origin in the red pulp during infection with the non-lethal parasite line P.yoelii 17X as opposed to infections with the P.yoelii 17XL lethal parasite line 7. To reach these conclusions, a specific methodology using ImageJ free software was developed to enable characterization of the fast three-dimensional movement of single-pRBCs. Results obtained with this protocol allow determining velocity, directionality and residence time of parasites in the spleen, all parameters addressing adherence in vivo. In addition, we report the methodology for blood flow quantification using intravital microscopy and the use of different colouring agents to gain insight into the complex microcirculatory structure of the spleen. 
Ethics statement
All the animal studies were performed at the animal facilities of University of Barcelona in accordance with guidelines and protocols approved by the Ethics Committee for Animal Experimentation of the University of Barcelona CEEA-UB (Protocol No DMAH: 5429). Female Balb/c mice of 6-8 weeks of age were obtained from Charles River Laboratories.

We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.

Microscopie intravitale de la rate: Analyse quantitative de la mobilité des parasites et la circulation sanguine

The advent of intravital microscopy in experimental rodent malaria models has allowed major advances to the knowledge of parasite-host interactions 1,2. ...

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Monitoring cellular communication by intravital deep-tissue multi-photon microscopy is the key for understanding the fate of immune cells within thick tissue samples and organs in health and disease. By controlling the scanning pattern in multi-photon microscopy and applying appropriate numerical algorithms, we developed a striped-illumination approach, which enabled us to achieve 3-fold better axial resolution and improved signal-to-noise ratio, i.e. contrast, in more than 100 &#181;m tissue depth within highly scattering tissue of lymphoid organs as compared to standard multi-photon microscopy. The acquisition speed as well as photobleaching and photodamage effects were similar to standard photo-multiplier-based technique, whereas the imaging depth was slightly lower due to the use of field detectors. By using the striped-illumination approach, we are able to observe the dynamics of immune complex deposits on secondary follicular dendritic cells &#8211; on the level of a few protein molecules in germinal centers.

High-resolution intravital imaging with enhanced contrast up to 120 &#181;m depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 &#181;m depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.

Très Résolu microscopie intravitale Striped-éclairage de centres germinaux

Monitoring cellular communication by intravital deep-tissue multi-photon microscopy is the key for understanding the fate of immune cells within thick ...

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Liver inflammation as a response to injury is a highly dynamic process involving the infiltration of distinct subtypes of leukocytes including monocytes, neutrophils, T cell subsets, B cells, natural killer (NK) and NKT cells. Intravital microscopy of the liver for monitoring immune cell migration is particularly challenging due to the high requirements regarding sample preparation and fixation, optical resolution and long-term animal survival. Yet, the dynamics of inflammatory processes as well as cellular interaction studies could provide critical information to better understand the initiation, progression and regression of inflammatory liver disease. Therefore, a highly sensitive and reliable method was established to study migration and cell-cell-interactions of different immune cells in mouse liver over long periods (about 6 hr) by intravital two-photon laser scanning microscopy (TPLSM) in combination with intensive care monitoring.
The method provided includes a gentle preparation and stable fixation of the liver with minimal perturbation of the organ; long term intravital imaging using multicolor multiphoton microscopy with virtually no photobleaching or phototoxic effects over a time period of up to 6 hr, allowing tracking of specific leukocyte subsets; and stable imaging conditions due to extensive monitoring of mouse vital parameters and stabilization of circulation, temperature and gas exchange.
To investigate lymphocyte migration upon liver inflammation CXCR6.gfp knock-in mice were subjected to intravital liver imaging under baseline conditions and after acute and chronic liver damage induced by intraperitoneal injection(s) of carbon tetrachloride (CCl4).
CXCR6 is a chemokine receptor expressed on lymphocytes, mainly on Natural Killer T (NKT)-, Natural Killer (NK)- and subsets of T lymphocytes such as CD4 T cells but also mucosal associated invariant (MAIT) T cells1. Following the migratory pattern and positioning of CXCR6.gfp+ immune cells allowed a detailed insight into their altered behavior upon liver injury and therefore their potential involvement in disease progression.

Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

À long terme intravitale microscopie multiphotonique imagerie des cellules immunitaires dans sains et malades du foie en utilisant des souris CXCR6.Gfp Reporter

Liver inflammation as a response to injury is a highly dynamic process involving the infiltration of distinct subtypes of leukocytes including monocytes, ...

Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

Efficient immune response is dependent on rapid mobilization of blood leukocytes to the site of infection or injury. Investigating leukocyte migration in vivo is crucial for understanding the molecular basis of leukocyte transendothelial migration and interaction with vascular endothelium. One powerful approach involves intravital microscopy on transgenic mice expressing fluorescent proteins in cells of interest.
Here we present a protocol for imaging monocytes and neutrophils in the CX3CR1gfp/wt mouse i.v. injected with orange dye-labeled neutrophils with an inverted confocal microscope. Time-lapse movies gathered from 30 min&#160;to several hours of imaging allow the analysis of leukocyte behavior in mesenteric veins under both steady state and inflammatory conditions. We also describe the steps to locally induce blood vessel inflammation with TLR2/TLR1 agonist Pam3SK4 and monitor the subsequent recruitment of neutrophils and monocytes.
The presented technique can also be used to monitor other populations of leukocytes and investigate molecules implicated in leukocyte recruitment or trafficking using other stimuli or transgenic mice.

We detail a protocol to monitor the behavior of neutrophils and monocytes in mesenteric veins under steady state and inflammatory conditions using intravital confocal microscopy on anaesthetized mice.

Imagerie neutrophiles et des monocytes dans les veines mésentérique par Microscopie intravitale sur des souris anesthésiées en temps réel

Efficient immune response is dependent on rapid mobilization of blood leukocytes to the site of infection or injury. Investigating leukocyte migration in ...

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

The submandibular salivary gland (SMG) is one of the three major salivary glands, and is of interest for many different fields of biological research, including cell biology, oncology, dentistry, and immunology. The SMG is an exocrine gland comprised of secretory epithelial cells, myofibroblasts, endothelial cells, nerves, and extracellular matrix. Dynamic cellular processes in the rat and mouse SMG have previously been imaged, mostly using inverted multi-photon microscope systems. Here, we describe a straightforward protocol for the surgical preparation and stabilization of the murine SMG in anesthetized mice for in vivo imaging with upright multi-photon microscope systems. We present representative intravital image sets of endogenous and adoptively transferred fluorescent cells, including the labeling of blood vessels or salivary ducts and second harmonic generation to visualize fibrillar collagen. In sum, our protocol allows for surgical preparation of mouse salivary glands in upright microscopy systems, which are commonly used for intravital imaging in the field of immunology.

We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.

Préparation du murin des glandes salivaires sous-maxillaires pour la microscopie intravitale verticale

The submandibular salivary gland (SMG) is one of the three major salivary glands, and is of interest for many different fields of biological research, ...

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy

The analysis of cell-cell or cell-pathogen interaction in vivo is an important tool to understand the dynamics of the immune response to infection. Two-photon intravital microscopy (2P-IVM) allows the observation of cell interactions in deep tissue in living animals, while minimizing the photobleaching generated during image acquisition. To date, different models for 2P-IVM of lymphoid and non-lymphoid organs have been described. However, imaging of respiratory organs remains a challenge due to the movement associated with the breathing cycle of the animal.
Here, we describe a protocol to visualize in vivo immune cell interactions in the trachea of mice infected with influenza virus using 2P-IVM. To this purpose, we developed a custom imaging platform, which included the surgical exposure and intubation of the trachea, followed by the acquisition of dynamic images of neutrophils and dendritic cells (DC) in the mucosal epithelium. Additionally, we detailed the steps needed to perform influenza intranasal infection and flow cytometric analysis of immune cells in the trachea. Finally, we analyzed neutrophil and DC motility as well as their interactions during the course of a movie. This protocol allows for the generation of stable and bright 4D images necessary for the assessment of cell-cell interactions in the trachea.

In this study, we present a protocol to perform two-photon intravital imaging and cell interaction analysis in the murine tracheal mucosa after infection with influenza virus. This protocol will be relevant for researchers studying immune cell dynamics during respiratory infections.

Imagerie cellulaire Interaction dans la muqueuse trachéale au cours de l’infection par le Virus grippal à l’aide de la microscopie intravitale biphotonique

The analysis of cell-cell or cell-pathogen interaction in vivo is an important tool to understand the dynamics of the immune response to infection. ...

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Human 3-dimensional (3D) enteroid or colonoid cultures derived from crypt base stem cells are currently the most advanced ex vivo model of the intestinal epithelium. Due to their closed structures and significant supporting extracellular matrix, 3D cultures are not ideal for host-pathogen studies. Enteroids or colonoids can be grown as epithelial monolayers on permeable tissue culture membranes to allow manipulation of both luminal and basolateral cell surfaces and accompanying fluids. This enhanced luminal surface accessibility facilitates modeling bacterial-host epithelial interactions such as the mucus-degrading ability of enterohemorrhagic E. coli (EHEC) on colonic epithelium. A method for 3D culture fragmentation, monolayer seeding, and transepithelial electrical resistance (TER) measurements to monitor the progress towards confluency and differentiation are described. Colonoid monolayer differentiation yields secreted mucus that can be studied by the immunofluorescence or immunoblotting techniques. More generally, enteroid or colonoid monolayers enable a physiologically-relevant platform to evaluate specific cell populations that may be targeted by pathogenic or commensal microbiota.

Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.

Monocouches de Colonoid humain pour étudier les Interactions entre les pathogènes, germes commensaux et hôte épithélium Intestinal

Human 3-dimensional (3D) enteroid or colonoid cultures derived from crypt base stem cells are currently the most advanced ex vivo model of the intestinal ...