Immunofluorescence and Linear Invadosome Quantification
7:26
3D Collagen Invasion Assay
11:19
Results
11:33
Conclusion
Transcription
Our group works on the interaction between cell and extracellular matrix. Cells can produce but also can degrade the extracellular matrix element. Degradation of the matrix can occur during physiological but also in pathological processes, such as
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This protocol describes how to prepare a 2D mixed matrix, consisting of gelatin and collagen I, and a 3D collagen I plug to study linear invadosomes. These protocols allow for the study of linear invadosome formation, matrix degradation activity, and the invasion capabilities of primary cells and cancer cell lines.