Flower and Silique Fixation and Dissection Under the Stereomicroscope
2:05
Chloral Hydrate-based Clearing and Combined Clearing-staining
3:28
Combined Alexander Staining and Herr's 4½ Clearing of Anthers
4:29
Removing the Exine from Pollen Grains
5:49
Sodium Dodecyl Sulfate (SDS) Clearing
7:04
Results: Arabidopsis Flowers and Floral Organs are Dissected, Cleared, and Stained for Whole Mount Observations of Reproductive Structures
8:41
Conclusion
Transcription
This protocol describes how to properly dissect flower buds, flowers and young siliques. And the subsequent whole-mounting clearing for diverse staining and observation techniques. This method can help answer key questions in the sexual plant prod
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In this protocol, we describe techniques for the proper dissection of Arabidopsis flowers and siliques, some basic clearing techniques, and selected staining procedures for whole-mount observations of reproductive structures.