Flower and Silique Fixation and Dissection Under the Stereomicroscope
2:05
Chloral Hydrate-based Clearing and Combined Clearing-staining
3:28
Combined Alexander Staining and Herr's 4½ Clearing of Anthers
4:29
Removing the Exine from Pollen Grains
5:49
Sodium Dodecyl Sulfate (SDS) Clearing
7:04
Results: Arabidopsis Flowers and Floral Organs are Dissected, Cleared, and Stained for Whole Mount Observations of Reproductive Structures
8:41
Conclusion
필기록
This protocol describes how to properly dissect flower buds, flowers and young siliques. And the subsequent whole-mounting clearing for diverse staining and observation techniques. This method can help answer key questions in the sexual plant prod
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
In this protocol, we describe techniques for the proper dissection of Arabidopsis flowers and siliques, some basic clearing techniques, and selected staining procedures for whole-mount observations of reproductive structures.