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Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

16.6K Views

09:17 min

April 12th, 2018

DOI :

10.3791/56441-v

April 12th, 2018


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Keywords Arabidopsis

Chapters in this video

0:04

Title

0:56

Flower and Silique Fixation and Dissection Under the Stereomicroscope

2:05

Chloral Hydrate-based Clearing and Combined Clearing-staining

3:28

Combined Alexander Staining and Herr's 4½ Clearing of Anthers

4:29

Removing the Exine from Pollen Grains

5:49

Sodium Dodecyl Sulfate (SDS) Clearing

7:04

Results: Arabidopsis Flowers and Floral Organs are Dissected, Cleared, and Stained for Whole Mount Observations of Reproductive Structures

8:41

Conclusion

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