The overall goal of this invasion assay is to quantify the migratory and invasive capacities of rare and sensitive cells in a 3D setting without dislodging the cells from their micro-environment. This method can help answer key questions in the ca
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This protocol describes an inverted vertical invasion assay that could be used to quantify the migration and invasion capabilities of cells in a three-dimensional setting while preserving the cell microenvironment. This assay is suitable for rare and/or sensitive cells.