Cell Transfection, Protein Purification, and Affinity Chromatrography
3:49
Bioactivity of IL4-10 Fusion Protein
4:46
Induction of Chronic Inflammatory Pain in Mice. Pain Measurements: The Von Frey Test: Mechanical Sensitivity
5:52
Pain measurements: Hargreaves Test for Thermal Sensitivity Dynamic Weight Bearing Test for Postural Deficits
6:48
Intrathecal Injection of IL4-10 Fusion Protein for Analgesia
7:56
Results: Persistent Inflammatory Pain Reduction Using IL4-10 Fusion Protein
9:38
Conclusion
Transcription
The overall goal of these procedures is to produce a fusion protein of the anti-inflammatory cytokines IL4 and IL10, control its quality, and evaluate its functional activity and analgesic properties in mirroring models of chronic inflammatory pai
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In this paper we provide details for the methods of production and quality control of an IL4-10 recombinant fusion protein. We also show how to test the effectiveness of this protein to resolve pain in a mouse model of inflammatory pain.