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JoVE Journal

Medicine

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Development of Recombinant Proteins to Treat Chronic Pain

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10:37 min

April 11th, 2018

April 11th, 2018

9,384 Views

0:05

Title

1:15

Cell Transfection, Protein Purification, and Affinity Chromatrography

3:49

Bioactivity of IL4-10 Fusion Protein

4:46

Induction of Chronic Inflammatory Pain in Mice. Pain Measurements: The Von Frey Test: Mechanical Sensitivity

5:52

Pain measurements: Hargreaves Test for Thermal Sensitivity Dynamic Weight Bearing Test for Postural Deficits

6:48

Intrathecal Injection of IL4-10 Fusion Protein for Analgesia

7:56

Results: Persistent Inflammatory Pain Reduction Using IL4-10 Fusion Protein

9:38

Conclusion

Transcrição

The overall goal of these procedures is to produce a fusion protein of the anti-inflammatory cytokines IL4 and IL10, control its quality, and evaluate its functional activity and analgesic properties in mirroring models of chronic inflammatory pai

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In this paper we provide details for the methods of production and quality control of an IL4-10 recombinant fusion protein. We also show how to test the effectiveness of this protein to resolve pain in a mouse model of inflammatory pain.

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