This method can help answer key questions about the composition of immune cell subsets within the heart both during steady and disease states. The main advantage of this technique is that it can be used for accurate immunophenotyping of cardiac im
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This protocol presents a simple and efficient method to isolate, identify and quantify immune cells residing in the myocardium of mice during steady state or inflammation. The protocol combines enzymatic and mechanical digestion for the generation of a single cell suspension that can be further analyzed by flow cytometry.