Solid-Phase Peptide Synthesis (SPPS), Peptide Cleavage from the Resin, and Purification of the Linear Precursor
3:04
Selective Formation of the Disulfide Bonds
4:44
Peptide Purification
5:56
Peptide Analytics
8:04
MS/MS Analysis of Disulfide Connectivity
9:49
Results: The Sequential Walk and the Resulting NMR Solution Structure of a Rigid and a Flexible -PIIIa Isomer
10:30
Conclusion
Transcription
This method can help answer key questions in the peptide synthesis field such as synthesizing disulfide rich peptides and determining their corresponding disulfide bridge pattern. The main advantage of this technique is that it allows for the sele
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Cysteine-rich peptides fold into distinct three-dimensional structures depending on their disulfide connectivity. Targeted synthesis of individual disulfide isomers is required when buffer oxidation does not lead to the desired disulfide connectivity. The protocol deals with the selective synthesis of 3-disulfide-bonded peptides and their structural analysis using NMR and MS/MS studies.