Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities
12.5K Views
•
11:44 min
•
October 2nd, 2018
DOI :
October 2nd, 2018
•Chapters in this video
0:04
Title
0:49
Solid-Phase Peptide Synthesis (SPPS), Peptide Cleavage from the Resin, and Purification of the Linear Precursor
3:04
Selective Formation of the Disulfide Bonds
4:44
Peptide Purification
5:56
Peptide Analytics
8:04
MS/MS Analysis of Disulfide Connectivity
9:49
Results: The Sequential Walk and the Resulting NMR Solution Structure of a Rigid and a Flexible -PIIIa Isomer
10:30
Conclusion
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