Scanning Fluorescence Cross-Correlation Spectroscopy (sFCCS): Data Analysis
6:03
Results: Investigation of Protein-Protein Interactions at Cell-Cell Contacts by sFCCS
8:09
Conclusion
Transcription
This method can help to understand key questions in the cell-to-cell interaction field such as which receptors mediate adhesion or recognition in cell-to-cell contacts and whether specific ligands trigger their interactions. The main advantage of
Connectez-vous ou commencez votre essai gratuit pour accéder à ce contenu.
This protocol describes a fluorescence fluctuation spectroscopy-based approach to investigate interactions among proteins mediating cell-cell interactions, i.e. proteins localized in cell junctions, directly in living cells. We provide detailed guidelines on instrument calibration, data acquisition and analysis, including corrections to possible artefact sources.