Fixation, Washing and Dehydration of Cyanobacteria and Euglenoids
2:36
Drying and Mounting
3:54
Fixation, Washing and Dehydration of Drosophila
4:55
Drosophila Drying and Mounting
6:48
Sputter Coating and Imaging
7:20
Results: Morphological Analysis of Different Organisms Using SEM Images
9:20
Conclusion
Transcription
This protocol focuses on two chemical drying methods, Hexamethyldisilazane and T-butyl alcohol and their application imaging both prokaryotic and eukaryotic organisms using SEM. The main advantage of this technique is that it does not require the
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SEM analysis is an effective method to aid in species identification or phenotypic discrimination. This protocol describes the methods for examining specific morphological details of three representative types of organisms and would be broadly applicable to examining features of many organismal and tissue types.