Sampling: Tension Relaxation within Water Column of Xylem Conduits
3:00
Freeze Fixation with Liquid Nitrogen
4:04
Specimen Preparation
5:49
Transfer to the Cryo-SEM Specimen Chamber
6:30
Freeze-etching
7:00
Results: Xylem Water Distribution in Woody Plants
9:04
Conclusion
Transcription
The overall goal of this protocol is providing us with electromicroscopy resolution of the water distribution in various kinds of xylem cells in situ. This method has been used to observe the water in xylem in order to clarify water distribution a
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Observing the water distribution within the xylem provides significant information regarding water flow dynamics in woody plants. In this study, we demonstrate the practical approach to observe xylem water distribution in situ by using a cryostat and cryo-SEM, which eliminates artifactual changes in the water status during sample preparation.