5-Bromo-2 -Deoxyuridine (BrdU) Quantification and Tissue Collectio
2:42
BrdU Immunoreactive Cell Area Measurement
4:23
Crypt Depth Measurement
4:57
Results: Representative Small Intestine Injury Recovery Assessment
6:17
Conclusion
Transcription
This model of chemotherapy-induced mucositis can be used to determine important endpoints and proliferated markers of small intestinal injury and compensatory hyperproliferation in mice. This method is time and cost efficient and easy to carry out
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Here, we present a protocol to establish important endpoints and proliferative markers of small intestinal injury and compensatory hyperproliferation using a model of chemotherapy-induced mucositis. We demonstrate the detection of proliferating cells using a cell cycle specific marker and using small intestinal weight, crypt depth, and villus height as endpoints.