Results: Quantifying Protein-RNA Binding in Bacteria
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Conclusion
Transcription
Characterization of protein binding to RNA in vivo remains a major challenge due to the complex nature of RNA and the many factors affecting its interaction with proteins. RNA binding protein or RBP-RNA affinity is measured inside live bacterial c
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In this method, we quantify the binding affinity of RNA binding proteins (RBPs) to cognate and non-cognate binding sites using a simple, live, reporter assay in bacterial cells. The assay is based on repression of a reporter gene.