Results: Representative Site-Specific Inflammatory mRNA and Protein Detection
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Conclusion
Transcription
Our protocol is an easy-to-use and inexpensive epidermal-dermal separation technique for determining the site-specific production of inflammatory mediators and neurotrophins during skin inflammation. The main advantage of this technique is that en
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Presented here is a protocol for the separation of epidermis from dermis to evaluate inflammatory mediator production. Following inflammation, rat hind paw epidermis is separated from the dermis by thermolysin at 4 °C. The epidermis is then used for mRNA analysis by RT-PCR and protein evaluation by western blot and immunohistochemistry.