This protocol, the in vitro invasion assay, is a useful tool for quantitatively measuring a cells lines ability to migrate through a protein rich matrix and pass through a porous membrane, in a process similar to the early stages of cancer cell in
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The in vitro cell invasion assay is used to measure the potential of cancer metastasis by quantifying the cellular potential for invasion and migration using cell culture inserts containing protein matrix. Cells are challenged to migrate through the protein matrix and a porous membrane, towards a chemoattractant, and then quantified by light microscopy.