Preparation of Poly-D-Lysine Plates for Neuron and Neurosphere Culture
2:38
Removal and Decapitation of the Fetus
3:28
Removal of Brain and Dissection of Cortex along with Hippocampus
4:32
Dissociation of Cortical and Hippocampal Tissues into Single Neurons
7:54
Results: Culture and Characterization of the Obtained Primary Neurons and Neurospheres
11:12
Conclusion
Transcription
The overall goal of this procedure is to demonstrate the development of neurospheres from mixed primary hippocampal and cortical neurons isolated from E14-E16 Sprague Dawley rat embryo, in order to offer a robust and low-cost platform for studying
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Presented here is a protocol for the spontaneous generation of neurospheres enriched in neural progenitor cells from high density plated neurons. During the same experiment, when neurons are plated at a lower density, the protocol also results in prolonged primary rat neuron cultures.