Reconstitution by Detergent Assisted Incorporation into Preformed Liposomes
5:10
Lipid Mixing Assays
6:16
Results: Analysis of Liposomes Reconstituted from Recombinant Drosophila Atlastin
7:58
Conclusion
Transcription
The reconstitution and fusion protocol detailed here is an efficient way to study membrane-bound proteins in a model lipid bilayer and lipid mixing by fusion proteins. Here we describe a detergent-assisted reconstitution of recombinant Drosophila
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Biological membrane fusion is catalyzed by specialized fusion proteins. Measuring the fusogenic properties of proteins can be achieved by lipid mixing assays. We present a method for purifying recombinant Drosophila atlastin, a protein that mediates homotypic fusion of the ER, reconstituting it to preformed liposomes, and testing for fusion capacity.