Yeast Induction and Sorting Nexin-Bin-Amphiphysin-Rvs (SNX-BAR) Dimer Purification
3:52
Liposome Preparation
6:44
SNX-BAR Liposome Binding and Tubulation
8:26
Results: Representative SNX-BAR Induction Verification, Purification, and Liposome Binding and Tubulation Assays
9:38
Conclusion
Transcription
SNX-BARs are membrane remodeling proteins that play key roles in human disease. Using our methods, we can determine their precise biophysical properties to facilitate lipid binding and membrane remodeling. Purifying the SNB-BAR proteins from yeast
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Here, we present a workflow for the expression, purification and liposome binding of SNX-BAR heterodimers in yeast.