There is a high number of EDC chemicals in our environment, mainly estrogenic substances. The chemical diversity of the substances belonging to the group makes their testing difficult, as different methods are required for their detection. Based o
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Present here is a detailed protocol for the use of zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects. The protocol covers the propagation of the fish and treatment of embryos, and emphasizes the detection, documentation, and the evaluation of fluorescent signals induced by endocrine disrupting compounds (EDC).