The overall goal of this microinjection of human breast cancer cells into embryonic zebrafish is to investigate the invasive behavior and assess the metastatic potential of human breast cancer cells. This method can help to answer key questions re
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Here, we describe xenograft zebrafish models using two different injection sites, i.e., perivitelline space and duct of Cuvier, to investigate the invasive behavior and to assess the intravasation and extravasation potential of human breast cancer cells, respectively.