Memory is a psychological process that entails various phases information acquisition, consolidation and retrieval. During the consolidation phase, establishment of new synaptic connections and modification of pre-existing connections occurs. This
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We describe an extended fear-conditioning protocol that produces overtraining and fear incubation in rats. This protocol entails a single training session with 25 tone-shock pairings (i.e., overtraining) and a comparison of conditioned freezing responses during context and cue tests 48 h (short-term) and 6 weeks (long-term) after training.