Kidney organ culture and organelles that are derived from programmed stem cells provide excellent ways to study mechanisms behind kidney development and disease. The current organ culture system do not provide blood flow to the kidney, however. Du
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This work describes two methods for studying organ development, an improved xenotransplantation setup on chorioallantoic membrane (CAM) from avian embryos that allows for vascularization of cultured embryonic organs and organoids and a novel fixed z-direction organ culture method with modified experimental conditions that allows for high-resolution time-lapse confocal imaging.