Lower Limb Vascular Blood Evaluation, Blood Sampling, and Balloon Angioplasty
4:07
Circulating Mononuclear Progenitor Cell (MPC) Isolation and Antibody Staining
5:21
MPC Quantification
6:10
Results: Representative Relationship Between MPC and Hemodynamic Indicators and Lower Limb Amputation Prognosis After Angioplasty
7:04
Conclusion
Transcription
Although angioplasty improves blood flow in critical limb ischemia, some patients will still progress to limb amputation. Compared to prognosis based on traditional risk factors and intrinsic vascular features, the number of local MPCs can be more
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Lower limb amputation may occur even after angioplasty of obstructed vessels in Critical Limb Ischemia (CLI). Mononuclear Progenitor Cells (MPCs) reflect vascular repair. The present protocol describes the quantification of MPCs from circulation close to angioplasty, and its relationship with endothelial dysfunction and prediction of lower limb amputation.