Lymphocyte Labeling and Experimental Setup for Microvascular Studies
4:40
Microcirculation of Lymphocytes
5:25
Results: Microscopic Images of Peyer s Patches from Rats
5:56
Conclusion
Transcription
This protocol makes it possible to perform in vivo observation of lymphocyte dynamics in rat Peyer's patches for long periods of time. It can be used to explore the effects of various tracks on lymphocytes in vivo. Demonstrating the procedure will
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Here, we describe a precise method for collecting thoracic duct lymphocytes and observing the migration of gut-tropic lymphocytes in rat Peyer’s patches for 3 hours using time-lapse photography. This technique can clarify how the dynamics of lymphocytes are affected under inflammatory conditions.