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Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-¹³C]Pyruvate and ¹³C/³¹P NMR Spectroscopy

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14:56 min

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April 21st, 2023

DOI :

10.3791/63188-v

April 21st, 2023

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David Shaul¹^,², Gal Sapir¹^,², Naama Lev-Cohain¹, Jacob Sosna¹, J. Moshe Gomori¹, Rachel Katz-Brull¹^,²

¹Department of Radiology, Hadassah Medical Organization and Faculty of Medicine, Hebrew University of Jerusalem, ²The Wohl Institute for Translational Medicine

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Hyperpolarized MRI

Cardiac Metabolism

Isolated Perfused Mouse Heart

Pyruvate Metabolism

13C 31P NMR Spectroscopy

Krebs Henseleit Buffer

Aerobic And Anaerobic Metabolism

Cardiac Energetics

Inorganic Phosphate

Perfusion System

Temperature Control

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Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-¹³C]Pyruvate and ¹³C/³¹P NMR Spectroscopy

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